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KMID : 0375319950170020297
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Journal of Clinical Pathology and Quality Control
1995 Volume.17 No. 2 p.297 ~ p.304
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Dual Cytochemieal Reaction of Combined Myeloperoxidase and Alpha-Naphthyl Butyrate Esterase (ANBE) Staining Method
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Abstract
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Background:
Dual cytochemical reaction is a method for combining two different stainings on a single slide. This method enables the simultaneous examination of leukemic cells exhibiting different substrate specificities. Most reports previously described
were
for the cytochemical reactions on combining specific and nonspecific esterase. To investigate the usefulness of dual myeloperoxidase staining, authors attempted to combine myeloperoxidase(MPO) and alpha-naphthyl butvrate esterase(ANBE) staining
methods.
Methods:
Bone marrow smear slides of patients with acute myelomonocytic leukemia and myelodysplastic syndrome were used for dual cytochemical reactions. Air dried slides were fixed with 0.5% CuSO4 for 1 minutes. Benzidine hydrogen peroxide solution
were
added for myeloperoxidase staining. Without counter staining procedures of safranin O, the same slides were immediately reacted with solution mixture of pararosanilin hydrochloride and alpha-naphthyl butyrate for 45 minutes. After washing in
distilled
water, slides were mounted with coverslip and examined under microscopy.
Results:
Granulocytic lineage cell stained dark blue by benzidine hydrogen peroxide reaction. Monocytic elements show reddish brown positivity in entire cytoplasm. The stainability and reaction intensity of dual staining were comparable to those of
ANBE
single staining.
Conclusions:
Dual myeloperoxidase staining method is helpful in simply identifying of cellular differentiation, especially myeloid and monocytic lineages on a single slide.
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KEYWORD
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